Perturbation¶

This guide demonstrates how to curate a complex, real world perturbation dataset McFarland et al. 2020 using the wetlab schema.

# !pip install 'lamindb[jupyter,aws,bionty]' wetlab
!lamin init --storage ./test-perturbation --schema bionty,wetlab

→ connected lamindb: testuser1/test-perturbation

import lamindb as ln
import bionty as bt
import wetlab as wl
import pandas as pd

pd.set_option("display.max_columns", None)

ln.context.uid = "K6sInKIQW5nt0003"
ln.context.track()

# See https://lamin.ai/laminlabs/lamindata/transform/13VINnFk89PE0006 to learn how this dataset was prepared
adata = ln.Artifact.using("laminlabs/lamindata").get(uid="Xk7Qaik9vBLV4PKf0001").load()
adata.obs.head(3)

Show code cell output Hide code cell output

→ completing transfer to track Artifact('Xk7Qaik9') as input

→ mapped records:

→ transferred records: Artifact(uid='Xk7Qaik9vBLV4PKf0001'), Storage(uid='D9BilDV2')

	depmap_id	cancer	cell_det_rate	cell_line	cell_quality	channel	disease	dose_unit	dose_value	doublet_CL1	doublet_CL2	doublet_GMM_prob	doublet_dev_imp	doublet_z_margin	hash_assignment	hash_tag	num_SNPs	organism	perturbation	perturbation_type	sex	singlet_ID	singlet_dev	singlet_dev_z	singlet_margin	singlet_z_margin	time	tissue_type	tot_reads	nperts	ngenes	ncounts	percent_mito	percent_ribo	chembl-ID
AACTGGTGTCTCTCTG	ACH-000390	True	0.093159	LUDLU-1	normal	nan	lung cancer	µM	0.1	LUDLU1_LUNG	TE14_OESOPHAGUS	2.269468e-10	0.009426	0.403316	nan	nan	481	human	trametinib	drug	Male	LUDLU1_LUNG	0.655877	14.860933	0.462273	12.351139	24	cell_line	787	1	3045	12895.0	3.202792	24.955409	CHEMBL2103875
ATAGGCTCAGATTTCG	ACH-000444	True	0.145728	LU99	normal	2	lung cancer	µM	0.5	LU99_LUNG	MCAS_OVARY	8.562908e-04	0.010173	0.188284	nan	nan	1003	human	afatinib	drug	Male	LU99_LUNG	0.762847	10.648094	0.474590	8.164565	24	cell_line	1597	1	4763	23161.0	7.473771	18.051898	CHEMBL1173655
GCCAAATCAAGCCGTC	ACH-000396	True	0.117330	J82	normal	nan	urinary bladder carcinoma	µM	0.1	J82_URINARY_TRACT	IGR1_SKIN	6.490367e-08	0.009686	1.185862	nan	nan	647	human	dabrafenib	drug	Male	J82_URINARY_TRACT	0.651059	14.740111	0.404508	11.188513	24	cell_line	1159	1	3834	18062.0	2.762706	22.085040	CHEMBL2028663

curate = ln.Curator.from_anndata(
    adata,
    var_index=bt.Gene.ensembl_gene_id,
    organism="human",
    using_key="laminlabs/lamindata"
)
curate.validate()

• 27 non-validated values are not saved in Feature.name: ['percent_mito', 'hash_assignment', 'singlet_dev', 'chembl-ID', 'cancer', 'nperts', 'percent_ribo', 'tot_reads', 'dose_value', 'num_SNPs', 'singlet_dev_z', 'cell_quality', 'hash_tag', 'singlet_z_margin', 'singlet_ID', 'doublet_z_margin', 'dose_unit', 'doublet_CL2', 'cell_det_rate', 'doublet_dev_imp', 'singlet_margin', 'ncounts', 'doublet_GMM_prob', 'doublet_CL1', 'channel', 'ngenes', 'depmap_id']!
      → to lookup values, use lookup().columns
      → to save, run add_new_from_columns

✓ added 8 records from laminlabs/lamindata with Feature.name for columns: 'tissue_type', 'perturbation_type', 'time', 'disease', 'sex', 'perturbation', 'organism', 'cell_line'

→ validating metadata using registries of instance laminlabs/lamindata

✓ created 1 Organism record from Bionty matching name: 'human'

• mapping var_index on Gene.ensembl_gene_id

!    found 1871 validated terms: ['ENSG00000102316', 'ENSG00000109472', 'ENSG00000080007', 'ENSG00000203926', 'ENSG00000232301', 'ENSG00000127419', 'ENSG00000266869', 'ENSG00000108960', 'ENSG00000261316', 'ENSG00000126870', 'ENSG00000121797', 'ENSG00000243927', 'ENSG00000143473', 'ENSG00000261488', 'ENSG00000115665', 'ENSG00000180613', 'ENSG00000167283', 'ENSG00000160472', 'ENSG00000273387', 'ENSG00000110768', 'ENSG00000253932', 'ENSG00000124507', 'ENSG00000258943', 'ENSG00000226698', 'ENSG00000259312', 'ENSG00000257381', 'ENSG00000129451', 'ENSG00000198743', 'ENSG00000228237', 'ENSG00000137033', 'ENSG00000120868', 'ENSG00000243410', 'ENSG00000079616', 'ENSG00000177082', 'ENSG00000233985', 'ENSG00000227392', 'ENSG00000251620', 'ENSG00000174804', 'ENSG00000057935', 'ENSG00000228204', 'ENSG00000251493', 'ENSG00000164182', 'ENSG00000112033', 'ENSG00000236388', 'ENSG00000116039', 'ENSG00000131095', 'ENSG00000170956', 'ENSG00000255289', 'ENSG00000237862', 'ENSG00000104870', 'ENSG00000259494', 'ENSG00000116906', 'ENSG00000173599', 'ENSG00000187080', 'ENSG00000167258', 'ENSG00000131473', 'ENSG00000107742', 'ENSG00000144791', 'ENSG00000198064', 'ENSG00000198286', 'ENSG00000196267', 'ENSG00000204791', 'ENSG00000165966', 'ENSG00000124383', 'ENSG00000154957', 'ENSG00000196689', 'ENSG00000133627', 'ENSG00000049656', 'ENSG00000266953', 'ENSG00000163918', 'ENSG00000163517', 'ENSG00000260478', 'ENSG00000166133', 'ENSG00000267052', 'ENSG00000003393', 'ENSG00000251438', 'ENSG00000186628', 'ENSG00000136002', 'ENSG00000244464', 'ENSG00000163629', 'ENSG00000262624', 'ENSG00000136319', 'ENSG00000273249', 'ENSG00000189159', 'ENSG00000266053', 'ENSG00000166770', 'ENSG00000167548', 'ENSG00000134780', 'ENSG00000257127', 'ENSG00000205571', 'ENSG00000262052', 'ENSG00000128309', 'ENSG00000111145', 'ENSG00000164587', 'ENSG00000096395', 'ENSG00000188820', 'ENSG00000148408', 'ENSG00000111554', 'ENSG00000140943', 'ENSG00000133101', 'ENSG00000148655', 'ENSG00000273312', 'ENSG00000073969', 'ENSG00000231671', 'ENSG00000198919', 'ENSG00000260773', 'ENSG00000256972', 'ENSG00000107331', 'ENSG00000120440', 'ENSG00000273329', 'ENSG00000267260', 'ENSG00000111801', 'ENSG00000267013', 'ENSG00000259060', 'ENSG00000117395', 'ENSG00000198938', 'ENSG00000204595', 'ENSG00000183304', 'ENSG00000165102', 'ENSG00000162460', 'ENSG00000272667', 'ENSG00000226041', 'ENSG00000150471', 'ENSG00000137634', 'ENSG00000106688', 'ENSG00000257543', 'ENSG00000254413', 'ENSG00000112282', 'ENSG00000203900', 'ENSG00000101146', 'ENSG00000162004', 'ENSG00000271576', 'ENSG00000233847', 'ENSG00000205293', 'ENSG00000175768', 'ENSG00000266013', 'ENSG00000171067', 'ENSG00000103365', 'ENSG00000206052', 'ENSG00000168288', 'ENSG00000237505', 'ENSG00000255008', 'ENSG00000240476', 'ENSG00000116833', 'ENSG00000237293', 'ENSG00000231949', 'ENSG00000111331', 'ENSG00000269927', 'ENSG00000215252', 'ENSG00000230569', 'ENSG00000143355', 'ENSG00000270800', 'ENSG00000161692', 'ENSG00000266389', 'ENSG00000089053', 'ENSG00000189007', 'ENSG00000182986', 'ENSG00000107859', 'ENSG00000099203', 'ENSG00000171861', 'ENSG00000125744', 'ENSG00000204880', 'ENSG00000239877', 'ENSG00000259132', 'ENSG00000229619', 'ENSG00000273155', 'ENSG00000166741', 'ENSG00000231720', 'ENSG00000223774', 'ENSG00000235478', 'ENSG00000251088', 'ENSG00000117139', 'ENSG00000153975', 'ENSG00000101188', 'ENSG00000146039', 'ENSG00000196666', 'ENSG00000256199', 'ENSG00000146733', 'ENSG00000251022', 'ENSG00000163218', 'ENSG00000169857', 'ENSG00000206432', 'ENSG00000128617', 'ENSG00000142173', 'ENSG00000179115', 'ENSG00000117318', 'ENSG00000131459', 'ENSG00000178403', 'ENSG00000224099', 'ENSG00000038295', 'ENSG00000148288', 'ENSG00000242612', 'ENSG00000179859', 'ENSG00000082781', 'ENSG00000230782', 'ENSG00000259283', 'ENSG00000234223', 'ENSG00000139684', 'ENSG00000186272', 'ENSG00000167604', 'ENSG00000268108', 'ENSG00000226398', 'ENSG00000235785', 'ENSG00000111049', 'ENSG00000261757', 'ENSG00000246740', 'ENSG00000149357', 'ENSG00000225867', 'ENSG00000132975', 'ENSG00000229258', 'ENSG00000166823', 'ENSG00000102871', 'ENSG00000205155', 'ENSG00000243550', 'ENSG00000183153', 'ENSG00000120438', 'ENSG00000232739', 'ENSG00000256810', 'ENSG00000157045', 'ENSG00000203280', 'ENSG00000262999', 'ENSG00000179751', 'ENSG00000255313', 'ENSG00000140259', 'ENSG00000269058', 'ENSG00000171817', 'ENSG00000130383', 'ENSG00000260793', 'ENSG00000256072', 'ENSG00000260526', 'ENSG00000234653', 'ENSG00000226649', 'ENSG00000179066', 'ENSG00000155438', 'ENSG00000152433', 'ENSG00000204463', 'ENSG00000146521', 'ENSG00000174950', 'ENSG00000269947', 'ENSG00000144741', 'ENSG00000174403', 'ENSG00000269275', 'ENSG00000062096', 'ENSG00000120656', 'ENSG00000009790', 'ENSG00000166037', 'ENSG00000245385', 'ENSG00000147650', 'ENSG00000127325', 'ENSG00000253433', 'ENSG00000139209', 'ENSG00000153317', 'ENSG00000132475', 'ENSG00000243818', 'ENSG00000225916', 'ENSG00000006453', 'ENSG00000229444', 'ENSG00000160813', 'ENSG00000138629', 'ENSG00000269846', 'ENSG00000166450', 'ENSG00000236665', 'ENSG00000175634', 'ENSG00000163254', 'ENSG00000258125', 'ENSG00000232555', 'ENSG00000261206', 'ENSG00000167608', 'ENSG00000259937', 'ENSG00000267160', 'ENSG00000213619', 'ENSG00000109171', 'ENSG00000230852', 'ENSG00000241764', 'ENSG00000249945', 'ENSG00000116685', 'ENSG00000251324', 'ENSG00000251258', 'ENSG00000062038', 'ENSG00000110497', 'ENSG00000069509', 'ENSG00000267779', 'ENSG00000249236', 'ENSG00000231944', 'ENSG00000269980', 'ENSG00000166171', 'ENSG00000254526', 'ENSG00000082512', 'ENSG00000162377', 'ENSG00000236311', 'ENSG00000146410', 'ENSG00000267686', 'ENSG00000186019', 'ENSG00000105369', 'ENSG00000137275', 'ENSG00000132846', 'ENSG00000262768', 'ENSG00000254910', 'ENSG00000160200', 'ENSG00000092148', 'ENSG00000184895', 'ENSG00000076351', 'ENSG00000011198', 'ENSG00000134531', 'ENSG00000163157', 'ENSG00000130584', 'ENSG00000223874', 'ENSG00000226321', 'ENSG00000174547', 'ENSG00000099994', 'ENSG00000146963', 'ENSG00000229557', 'ENSG00000197683', 'ENSG00000185033', 'ENSG00000175548', 'ENSG00000072133', 'ENSG00000205085', 'ENSG00000228478', 'ENSG00000125695', 'ENSG00000137040', 'ENSG00000251033', 'ENSG00000180138', 'ENSG00000173905', 'ENSG00000137166', 'ENSG00000259588', 'ENSG00000177294', 'ENSG00000226853', 'ENSG00000114942', 'ENSG00000256875', 'ENSG00000112658', 'ENSG00000150594', 'ENSG00000164631', 'ENSG00000151650', 'ENSG00000223638', 'ENSG00000259925', 'ENSG00000229086', 'ENSG00000198542', 'ENSG00000196436', 'ENSG00000224265', 'ENSG00000175854', 'ENSG00000226204', 'ENSG00000231705', 'ENSG00000255181', 'ENSG00000101004', 'ENSG00000217930', 'ENSG00000077935', 'ENSG00000223697', 'ENSG00000172340', 'ENSG00000023191', 'ENSG00000189164', 'ENSG00000177990', 'ENSG00000179873', 'ENSG00000187772', 'ENSG00000155729', 'ENSG00000230159', 'ENSG00000124440', 'ENSG00000142694', 'ENSG00000105875', 'ENSG00000158828', 'ENSG00000111371', 'ENSG00000159337', 'ENSG00000176153', 'ENSG00000168702', 'ENSG00000256632', 'ENSG00000235718', 'ENSG00000106588', 'ENSG00000134873', 'ENSG00000141096', 'ENSG00000116786', 'ENSG00000244650', 'ENSG00000054938', 'ENSG00000239225', 'ENSG00000086967', 'ENSG00000171431', 'ENSG00000188263', 'ENSG00000170807', 'ENSG00000007129', 'ENSG00000256151', 'ENSG00000168234', 'ENSG00000173141', 'ENSG00000244395', 'ENSG00000100181', 'ENSG00000235724', 'ENSG00000118898', 'ENSG00000232949', 'ENSG00000235263', 'ENSG00000268745', 'ENSG00000185960', 'ENSG00000236141', 'ENSG00000168269', 'ENSG00000084112', 'ENSG00000237063', 'ENSG00000096872', 'ENSG00000261325', 'ENSG00000175066', 'ENSG00000224846', 'ENSG00000203952', 'ENSG00000196767', 'ENSG00000138381', 'ENSG00000233223', 'ENSG00000262714', 'ENSG00000271717', 'ENSG00000142661', 'ENSG00000228959', 'ENSG00000198815', 'ENSG00000204538', 'ENSG00000253275', 'ENSG00000118200', 'ENSG00000147655', 'ENSG00000145194', 'ENSG00000213088', 'ENSG00000177272', 'ENSG00000126464', 'ENSG00000175087', 'ENSG00000011021', 'ENSG00000125834', 'ENSG00000197766', 'ENSG00000247765', 'ENSG00000248710', 'ENSG00000268203', 'ENSG00000119514', 'ENSG00000165471', 'ENSG00000172469', 'ENSG00000176142', 'ENSG00000235410', 'ENSG00000173786', 'ENSG00000247473', 'ENSG00000119943', 'ENSG00000272798', 'ENSG00000188342', 'ENSG00000121481', 'ENSG00000080815', 'ENSG00000165506', 'ENSG00000248373', 'ENSG00000269091', 'ENSG00000161835', 'ENSG00000184117', 'ENSG00000188649', 'ENSG00000114480', 'ENSG00000259819', 'ENSG00000268573', 'ENSG00000134333', 'ENSG00000110900', 'ENSG00000082293', 'ENSG00000151640', 'ENSG00000059377', 'ENSG00000253503', 'ENSG00000125514', 'ENSG00000179743', 'ENSG00000148468', 'ENSG00000148110', 'ENSG00000039139', 'ENSG00000268400', 'ENSG00000116151', 'ENSG00000197620', 'ENSG00000154025', 'ENSG00000151690', 'ENSG00000255823', 'ENSG00000272425', 'ENSG00000188523', 'ENSG00000255517', 'ENSG00000196961', 'ENSG00000048740', 'ENSG00000225473', 'ENSG00000020256', 'ENSG00000106683', 'ENSG00000225233', 'ENSG00000233987', 'ENSG00000105204', 'ENSG00000136352', 'ENSG00000261754', 'ENSG00000183281', 'ENSG00000254377', 'ENSG00000272843', 'ENSG00000180596', 'ENSG00000138347', 'ENSG00000157335', 'ENSG00000227051', 'ENSG00000187624', 'ENSG00000231418', 'ENSG00000244462', 'ENSG00000250091', 'ENSG00000142207', 'ENSG00000164663', 'ENSG00000224655', 'ENSG00000003756', 'ENSG00000170523', 'ENSG00000141431', 'ENSG00000203279', 'ENSG00000268673', 'ENSG00000106404', 'ENSG00000254656', 'ENSG00000254438', 'ENSG00000255502', 'ENSG00000174348', 'ENSG00000251326', 'ENSG00000130827', 'ENSG00000145391', 'ENSG00000169499', 'ENSG00000250415', 'ENSG00000233349', 'ENSG00000130700', 'ENSG00000159128', 'ENSG00000232811', 'ENSG00000242715', 'ENSG00000140526', 'ENSG00000212747', 'ENSG00000254306', 'ENSG00000243795', 'ENSG00000232600', 'ENSG00000214827', 'ENSG00000234945', 'ENSG00000181982', 'ENSG00000223914', 'ENSG00000204351', 'ENSG00000271119', 'ENSG00000185261', 'ENSG00000151388', 'ENSG00000184345', 'ENSG00000223462', 'ENSG00000271434', 'ENSG00000172782', 'ENSG00000255462', 'ENSG00000005302', 'ENSG00000127515', 'ENSG00000261734', 'ENSG00000136938', 'ENSG00000169750', 'ENSG00000213588', 'ENSG00000229821', 'ENSG00000186862', 'ENSG00000151445', 'ENSG00000106609', 'ENSG00000120436', 'ENSG00000256551', 'ENSG00000180872', 'ENSG00000116977', 'ENSG00000100522', 'ENSG00000133706', 'ENSG00000248920', 'ENSG00000184436', 'ENSG00000233221', 'ENSG00000259209', 'ENSG00000125247', 'ENSG00000164621', 'ENSG00000104218', 'ENSG00000111834', 'ENSG00000234323', 'ENSG00000260286', 'ENSG00000228526', 'ENSG00000082068', 'ENSG00000205111', 'ENSG00000166261', 'ENSG00000197580', 'ENSG00000225819', 'ENSG00000168214', 'ENSG00000231373', 'ENSG00000255311', 'ENSG00000018625', 'ENSG00000236637', 'ENSG00000165006', 'ENSG00000113575', 'ENSG00000140396', 'ENSG00000225606', 'ENSG00000145337', 'ENSG00000135677', 'ENSG00000151379', 'ENSG00000229679', 'ENSG00000169155', 'ENSG00000254846', 'ENSG00000164761', 'ENSG00000234527', 'ENSG00000232465', 'ENSG00000149633', 'ENSG00000079974', 'ENSG00000225519', 'ENSG00000235303', 'ENSG00000229603', 'ENSG00000228587', 'ENSG00000108474', 'ENSG00000103061', 'ENSG00000253457', 'ENSG00000272370', 'ENSG00000120057', 'ENSG00000176230', 'ENSG00000246130', 'ENSG00000262903', 'ENSG00000043514', 'ENSG00000167483', 'ENSG00000145781', 'ENSG00000134775', 'ENSG00000086991', 'ENSG00000138107', 'ENSG00000255039', 'ENSG00000149150', 'ENSG00000003989', 'ENSG00000063438', 'ENSG00000175643', 'ENSG00000198062', 'ENSG00000183470', 'ENSG00000188766', 'ENSG00000270082', 'ENSG00000089094', 'ENSG00000263635', 'ENSG00000109814', 'ENSG00000173467', 'ENSG00000110066', 'ENSG00000125879', 'ENSG00000004838', 'ENSG00000227704', 'ENSG00000109113', 'ENSG00000247877', 'ENSG00000120594', 'ENSG00000108094', 'ENSG00000169016', 'ENSG00000125148', 'ENSG00000259181', 'ENSG00000257591', 'ENSG00000242689', 'ENSG00000141198', 'ENSG00000080709', 'ENSG00000142864', 'ENSG00000156675', 'ENSG00000157557', 'ENSG00000148215', 'ENSG00000230401', 'ENSG00000111247', 'ENSG00000227706', 'ENSG00000173715', 'ENSG00000205220', 'ENSG00000213799', 'ENSG00000173702', 'ENSG00000188729', 'ENSG00000099800', 'ENSG00000181555', 'ENSG00000234684', 'ENSG00000111481', 'ENSG00000232987', 'ENSG00000185630', 'ENSG00000136840', 'ENSG00000258444', 'ENSG00000164047', 'ENSG00000228661', 'ENSG00000139726', 'ENSG00000243477', 'ENSG00000259044', 'ENSG00000183401', 'ENSG00000168993', 'ENSG00000239839', 'ENSG00000233941', 'ENSG00000257435', 'ENSG00000179284', 'ENSG00000140992', 'ENSG00000135363', 'ENSG00000255420', 'ENSG00000115425', 'ENSG00000121380', 'ENSG00000225537', 'ENSG00000119041', 'ENSG00000123243', 'ENSG00000254863', 'ENSG00000139192', 'ENSG00000165632', 'ENSG00000230768', 'ENSG00000114054', 'ENSG00000049860', 'ENSG00000248698', 'ENSG00000213468', 'ENSG00000168591', 'ENSG00000132561', 'ENSG00000256286', 'ENSG00000139180', 'ENSG00000259456', 'ENSG00000255666', 'ENSG00000166987', 'ENSG00000167384', 'ENSG00000156140', 'ENSG00000119686', 'ENSG00000228567', 'ENSG00000237707', 'ENSG00000182810', 'ENSG00000234230', 'ENSG00000258858', 'ENSG00000256803', 'ENSG00000125144', 'ENSG00000126775', 'ENSG00000240770', 'ENSG00000205126', 'ENSG00000254120', 'ENSG00000172840', 'ENSG00000103381', 'ENSG00000178057', 'ENSG00000108064', 'ENSG00000120322', 'ENSG00000226996', 'ENSG00000214970', 'ENSG00000255663', 'ENSG00000227459', 'ENSG00000250948', 'ENSG00000139428', 'ENSG00000173473', 'ENSG00000231902', 'ENSG00000247903', 'ENSG00000229242', 'ENSG00000181458', 'ENSG00000101474', 'ENSG00000165209', 'ENSG00000046651', 'ENSG00000268061', 'ENSG00000221963', 'ENSG00000165325', 'ENSG00000100884', 'ENSG00000238189', 'ENSG00000234721', 'ENSG00000241962', 'ENSG00000148803', 'ENSG00000175206', 'ENSG00000248107', 'ENSG00000272277', 'ENSG00000120805', 'ENSG00000049249', 'ENSG00000090776', 'ENSG00000070731', 'ENSG00000148339', 'ENSG00000113194', 'ENSG00000110628', 'ENSG00000147873', 'ENSG00000164600', 'ENSG00000126705', 'ENSG00000235237', 'ENSG00000196502', 'ENSG00000154473', 'ENSG00000143921', 'ENSG00000105609', 'ENSG00000263924', 'ENSG00000184076', 'ENSG00000177822', 'ENSG00000261583', 'ENSG00000119283', 'ENSG00000235760', 'ENSG00000196361', 'ENSG00000109775', 'ENSG00000266369', 'ENSG00000179869', 'ENSG00000258285', 'ENSG00000123213', 'ENSG00000169836', 'ENSG00000184650', 'ENSG00000241151', 'ENSG00000138459', 'ENSG00000248576', 'ENSG00000235481', 'ENSG00000163734', 'ENSG00000106028', 'ENSG00000253298', 'ENSG00000259865', 'ENSG00000100652', 'ENSG00000251442', 'ENSG00000167491', 'ENSG00000258631', 'ENSG00000170748', 'ENSG00000166965', 'ENSG00000203661', 'ENSG00000108061', 'ENSG00000203857', 'ENSG00000131398', 'ENSG00000143632', 'ENSG00000258130', 'ENSG00000167914', 'ENSG00000221988', 'ENSG00000266728', 'ENSG00000246016', 'ENSG00000139330', 'ENSG00000101773', 'ENSG00000233718', 'ENSG00000204481', 'ENSG00000173918', 'ENSG00000268707', 'ENSG00000234186', 'ENSG00000233534', 'ENSG00000186451', 'ENSG00000114124', 'ENSG00000178741', 'ENSG00000249776', 'ENSG00000196407', 'ENSG00000228358', 'ENSG00000261396', 'ENSG00000119986', 'ENSG00000179038', 'ENSG00000161551', 'ENSG00000105698', 'ENSG00000164756', 'ENSG00000232640', 'ENSG00000205403', 'ENSG00000115091', 'ENSG00000173208', 'ENSG00000236874', 'ENSG00000197651', 'ENSG00000270403', 'ENSG00000203721', 'ENSG00000156253', 'ENSG00000231393', 'ENSG00000230133', 'ENSG00000179755', 'ENSG00000196341', 'ENSG00000131116', 'ENSG00000141696', 'ENSG00000081692', 'ENSG00000126368', 'ENSG00000088002', 'ENSG00000090266', 'ENSG00000162909', 'ENSG00000228763', 'ENSG00000186684', 'ENSG00000188038', 'ENSG00000254477', 'ENSG00000153446', 'ENSG00000251144', 'ENSG00000106771', 'ENSG00000078295', 'ENSG00000223675', 'ENSG00000229116', 'ENSG00000112053', 'ENSG00000117153', 'ENSG00000198843', 'ENSG00000164010', 'ENSG00000144120', 'ENSG00000187581', 'ENSG00000269300', 'ENSG00000070770', 'ENSG00000148341', 'ENSG00000261798', 'ENSG00000230149', 'ENSG00000146360', 'ENSG00000137936', 'ENSG00000123338', 'ENSG00000120733', 'ENSG00000258902', 'ENSG00000139154', 'ENSG00000268654', 'ENSG00000254415', 'ENSG00000260907', 'ENSG00000254043', 'ENSG00000243636', 'ENSG00000198755', 'ENSG00000101435', 'ENSG00000118369', 'ENSG00000189067', 'ENSG00000267761', 'ENSG00000118004', 'ENSG00000160808', 'ENSG00000178988', 'ENSG00000179144', 'ENSG00000184814', 'ENSG00000171634', 'ENSG00000136682', 'ENSG00000114544', 'ENSG00000245888', 'ENSG00000259776', 'ENSG00000115008', 'ENSG00000153246', 'ENSG00000269858', 'ENSG00000237357', 'ENSG00000159212', 'ENSG00000249153', 'ENSG00000214842', 'ENSG00000134757', 'ENSG00000131849', 'ENSG00000157087', 'ENSG00000236427', 'ENSG00000250555', 'ENSG00000268658', 'ENSG00000205129', 'ENSG00000212856', 'ENSG00000164002', 'ENSG00000273133', 'ENSG00000147099', 'ENSG00000100321', 'ENSG00000163528', 'ENSG00000215403', 'ENSG00000165113', 'ENSG00000137522', 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'ENSG00000179044']
      → save terms via .add_validated_from_var_index()

False

# The cells were subject to several types of perturbations that we will curate separately
adata.obs.perturbation_type.value_counts()

Curate non-perturbation metadata¶

categoricals = {
    "depmap_id": bt.CellLine.ontology_id,
    "cell_line": bt.CellLine.name,
    "disease": bt.Disease.name,
    "organism": bt.Organism.name,
    "perturbation_type": ln.ULabel.name,
    "sex": bt.Phenotype.name,
    "time": ln.ULabel.name,
    "tissue_type": ln.ULabel.name,
}
sources = {
    "depmap_id": bt.Source.using("laminlabs/lamindata").filter(name="depmap").one(),
    "cell_line": bt.Source.using("laminlabs/lamindata").filter(name="depmap").one(),
}

curate = ln.Curator.from_anndata(
    adata,
    var_index=bt.Gene.ensembl_gene_id,
    categoricals=categoricals,
    organism="human",
    sources=sources,
    using_key="laminlabs/lamindata"
)

curate.validate()

Show code cell output Hide code cell output

✓    added 1 record with Feature.name for columns: 'depmap_id'

•    26 non-validated values are not saved in Feature.name: ['percent_mito', 'hash_assignment', 'singlet_dev', 'chembl-ID', 'cancer', 'nperts', 'percent_ribo', 'tot_reads', 'dose_value', 'num_SNPs', 'singlet_dev_z', 'cell_quality', 'hash_tag', 'singlet_z_margin', 'singlet_ID', 'doublet_z_margin', 'dose_unit', 'doublet_CL2', 'cell_det_rate', 'doublet_dev_imp', 'singlet_margin', 'ncounts', 'doublet_GMM_prob', 'doublet_CL1', 'channel', 'ngenes']!
      → to lookup values, use lookup().columns
      → to save, run add_new_from_columns

→    validating metadata using registries of instance laminlabs/lamindata

• mapping var_index on Gene.ensembl_gene_id

!    found 1871 validated terms: ['ENSG00000102316', 'ENSG00000109472', 'ENSG00000080007', 'ENSG00000203926', 'ENSG00000232301', 'ENSG00000127419', 'ENSG00000266869', 'ENSG00000108960', 'ENSG00000261316', 'ENSG00000126870', 'ENSG00000121797', 'ENSG00000243927', 'ENSG00000143473', 'ENSG00000261488', 'ENSG00000115665', 'ENSG00000180613', 'ENSG00000167283', 'ENSG00000160472', 'ENSG00000273387', 'ENSG00000110768', 'ENSG00000253932', 'ENSG00000124507', 'ENSG00000258943', 'ENSG00000226698', 'ENSG00000259312', 'ENSG00000257381', 'ENSG00000129451', 'ENSG00000198743', 'ENSG00000228237', 'ENSG00000137033', 'ENSG00000120868', 'ENSG00000243410', 'ENSG00000079616', 'ENSG00000177082', 'ENSG00000233985', 'ENSG00000227392', 'ENSG00000251620', 'ENSG00000174804', 'ENSG00000057935', 'ENSG00000228204', 'ENSG00000251493', 'ENSG00000164182', 'ENSG00000112033', 'ENSG00000236388', 'ENSG00000116039', 'ENSG00000131095', 'ENSG00000170956', 'ENSG00000255289', 'ENSG00000237862', 'ENSG00000104870', 'ENSG00000259494', 'ENSG00000116906', 'ENSG00000173599', 'ENSG00000187080', 'ENSG00000167258', 'ENSG00000131473', 'ENSG00000107742', 'ENSG00000144791', 'ENSG00000198064', 'ENSG00000198286', 'ENSG00000196267', 'ENSG00000204791', 'ENSG00000165966', 'ENSG00000124383', 'ENSG00000154957', 'ENSG00000196689', 'ENSG00000133627', 'ENSG00000049656', 'ENSG00000266953', 'ENSG00000163918', 'ENSG00000163517', 'ENSG00000260478', 'ENSG00000166133', 'ENSG00000267052', 'ENSG00000003393', 'ENSG00000251438', 'ENSG00000186628', 'ENSG00000136002', 'ENSG00000244464', 'ENSG00000163629', 'ENSG00000262624', 'ENSG00000136319', 'ENSG00000273249', 'ENSG00000189159', 'ENSG00000266053', 'ENSG00000166770', 'ENSG00000167548', 'ENSG00000134780', 'ENSG00000257127', 'ENSG00000205571', 'ENSG00000262052', 'ENSG00000128309', 'ENSG00000111145', 'ENSG00000164587', 'ENSG00000096395', 'ENSG00000188820', 'ENSG00000148408', 'ENSG00000111554', 'ENSG00000140943', 'ENSG00000133101', 'ENSG00000148655', 'ENSG00000273312', 'ENSG00000073969', 'ENSG00000231671', 'ENSG00000198919', 'ENSG00000260773', 'ENSG00000256972', 'ENSG00000107331', 'ENSG00000120440', 'ENSG00000273329', 'ENSG00000267260', 'ENSG00000111801', 'ENSG00000267013', 'ENSG00000259060', 'ENSG00000117395', 'ENSG00000198938', 'ENSG00000204595', 'ENSG00000183304', 'ENSG00000165102', 'ENSG00000162460', 'ENSG00000272667', 'ENSG00000226041', 'ENSG00000150471', 'ENSG00000137634', 'ENSG00000106688', 'ENSG00000257543', 'ENSG00000254413', 'ENSG00000112282', 'ENSG00000203900', 'ENSG00000101146', 'ENSG00000162004', 'ENSG00000271576', 'ENSG00000233847', 'ENSG00000205293', 'ENSG00000175768', 'ENSG00000266013', 'ENSG00000171067', 'ENSG00000103365', 'ENSG00000206052', 'ENSG00000168288', 'ENSG00000237505', 'ENSG00000255008', 'ENSG00000240476', 'ENSG00000116833', 'ENSG00000237293', 'ENSG00000231949', 'ENSG00000111331', 'ENSG00000269927', 'ENSG00000215252', 'ENSG00000230569', 'ENSG00000143355', 'ENSG00000270800', 'ENSG00000161692', 'ENSG00000266389', 'ENSG00000089053', 'ENSG00000189007', 'ENSG00000182986', 'ENSG00000107859', 'ENSG00000099203', 'ENSG00000171861', 'ENSG00000125744', 'ENSG00000204880', 'ENSG00000239877', 'ENSG00000259132', 'ENSG00000229619', 'ENSG00000273155', 'ENSG00000166741', 'ENSG00000231720', 'ENSG00000223774', 'ENSG00000235478', 'ENSG00000251088', 'ENSG00000117139', 'ENSG00000153975', 'ENSG00000101188', 'ENSG00000146039', 'ENSG00000196666', 'ENSG00000256199', 'ENSG00000146733', 'ENSG00000251022', 'ENSG00000163218', 'ENSG00000169857', 'ENSG00000206432', 'ENSG00000128617', 'ENSG00000142173', 'ENSG00000179115', 'ENSG00000117318', 'ENSG00000131459', 'ENSG00000178403', 'ENSG00000224099', 'ENSG00000038295', 'ENSG00000148288', 'ENSG00000242612', 'ENSG00000179859', 'ENSG00000082781', 'ENSG00000230782', 'ENSG00000259283', 'ENSG00000234223', 'ENSG00000139684', 'ENSG00000186272', 'ENSG00000167604', 'ENSG00000268108', 'ENSG00000226398', 'ENSG00000235785', 'ENSG00000111049', 'ENSG00000261757', 'ENSG00000246740', 'ENSG00000149357', 'ENSG00000225867', 'ENSG00000132975', 'ENSG00000229258', 'ENSG00000166823', 'ENSG00000102871', 'ENSG00000205155', 'ENSG00000243550', 'ENSG00000183153', 'ENSG00000120438', 'ENSG00000232739', 'ENSG00000256810', 'ENSG00000157045', 'ENSG00000203280', 'ENSG00000262999', 'ENSG00000179751', 'ENSG00000255313', 'ENSG00000140259', 'ENSG00000269058', 'ENSG00000171817', 'ENSG00000130383', 'ENSG00000260793', 'ENSG00000256072', 'ENSG00000260526', 'ENSG00000234653', 'ENSG00000226649', 'ENSG00000179066', 'ENSG00000155438', 'ENSG00000152433', 'ENSG00000204463', 'ENSG00000146521', 'ENSG00000174950', 'ENSG00000269947', 'ENSG00000144741', 'ENSG00000174403', 'ENSG00000269275', 'ENSG00000062096', 'ENSG00000120656', 'ENSG00000009790', 'ENSG00000166037', 'ENSG00000245385', 'ENSG00000147650', 'ENSG00000127325', 'ENSG00000253433', 'ENSG00000139209', 'ENSG00000153317', 'ENSG00000132475', 'ENSG00000243818', 'ENSG00000225916', 'ENSG00000006453', 'ENSG00000229444', 'ENSG00000160813', 'ENSG00000138629', 'ENSG00000269846', 'ENSG00000166450', 'ENSG00000236665', 'ENSG00000175634', 'ENSG00000163254', 'ENSG00000258125', 'ENSG00000232555', 'ENSG00000261206', 'ENSG00000167608', 'ENSG00000259937', 'ENSG00000267160', 'ENSG00000213619', 'ENSG00000109171', 'ENSG00000230852', 'ENSG00000241764', 'ENSG00000249945', 'ENSG00000116685', 'ENSG00000251324', 'ENSG00000251258', 'ENSG00000062038', 'ENSG00000110497', 'ENSG00000069509', 'ENSG00000267779', 'ENSG00000249236', 'ENSG00000231944', 'ENSG00000269980', 'ENSG00000166171', 'ENSG00000254526', 'ENSG00000082512', 'ENSG00000162377', 'ENSG00000236311', 'ENSG00000146410', 'ENSG00000267686', 'ENSG00000186019', 'ENSG00000105369', 'ENSG00000137275', 'ENSG00000132846', 'ENSG00000262768', 'ENSG00000254910', 'ENSG00000160200', 'ENSG00000092148', 'ENSG00000184895', 'ENSG00000076351', 'ENSG00000011198', 'ENSG00000134531', 'ENSG00000163157', 'ENSG00000130584', 'ENSG00000223874', 'ENSG00000226321', 'ENSG00000174547', 'ENSG00000099994', 'ENSG00000146963', 'ENSG00000229557', 'ENSG00000197683', 'ENSG00000185033', 'ENSG00000175548', 'ENSG00000072133', 'ENSG00000205085', 'ENSG00000228478', 'ENSG00000125695', 'ENSG00000137040', 'ENSG00000251033', 'ENSG00000180138', 'ENSG00000173905', 'ENSG00000137166', 'ENSG00000259588', 'ENSG00000177294', 'ENSG00000226853', 'ENSG00000114942', 'ENSG00000256875', 'ENSG00000112658', 'ENSG00000150594', 'ENSG00000164631', 'ENSG00000151650', 'ENSG00000223638', 'ENSG00000259925', 'ENSG00000229086', 'ENSG00000198542', 'ENSG00000196436', 'ENSG00000224265', 'ENSG00000175854', 'ENSG00000226204', 'ENSG00000231705', 'ENSG00000255181', 'ENSG00000101004', 'ENSG00000217930', 'ENSG00000077935', 'ENSG00000223697', 'ENSG00000172340', 'ENSG00000023191', 'ENSG00000189164', 'ENSG00000177990', 'ENSG00000179873', 'ENSG00000187772', 'ENSG00000155729', 'ENSG00000230159', 'ENSG00000124440', 'ENSG00000142694', 'ENSG00000105875', 'ENSG00000158828', 'ENSG00000111371', 'ENSG00000159337', 'ENSG00000176153', 'ENSG00000168702', 'ENSG00000256632', 'ENSG00000235718', 'ENSG00000106588', 'ENSG00000134873', 'ENSG00000141096', 'ENSG00000116786', 'ENSG00000244650', 'ENSG00000054938', 'ENSG00000239225', 'ENSG00000086967', 'ENSG00000171431', 'ENSG00000188263', 'ENSG00000170807', 'ENSG00000007129', 'ENSG00000256151', 'ENSG00000168234', 'ENSG00000173141', 'ENSG00000244395', 'ENSG00000100181', 'ENSG00000235724', 'ENSG00000118898', 'ENSG00000232949', 'ENSG00000235263', 'ENSG00000268745', 'ENSG00000185960', 'ENSG00000236141', 'ENSG00000168269', 'ENSG00000084112', 'ENSG00000237063', 'ENSG00000096872', 'ENSG00000261325', 'ENSG00000175066', 'ENSG00000224846', 'ENSG00000203952', 'ENSG00000196767', 'ENSG00000138381', 'ENSG00000233223', 'ENSG00000262714', 'ENSG00000271717', 'ENSG00000142661', 'ENSG00000228959', 'ENSG00000198815', 'ENSG00000204538', 'ENSG00000253275', 'ENSG00000118200', 'ENSG00000147655', 'ENSG00000145194', 'ENSG00000213088', 'ENSG00000177272', 'ENSG00000126464', 'ENSG00000175087', 'ENSG00000011021', 'ENSG00000125834', 'ENSG00000197766', 'ENSG00000247765', 'ENSG00000248710', 'ENSG00000268203', 'ENSG00000119514', 'ENSG00000165471', 'ENSG00000172469', 'ENSG00000176142', 'ENSG00000235410', 'ENSG00000173786', 'ENSG00000247473', 'ENSG00000119943', 'ENSG00000272798', 'ENSG00000188342', 'ENSG00000121481', 'ENSG00000080815', 'ENSG00000165506', 'ENSG00000248373', 'ENSG00000269091', 'ENSG00000161835', 'ENSG00000184117', 'ENSG00000188649', 'ENSG00000114480', 'ENSG00000259819', 'ENSG00000268573', 'ENSG00000134333', 'ENSG00000110900', 'ENSG00000082293', 'ENSG00000151640', 'ENSG00000059377', 'ENSG00000253503', 'ENSG00000125514', 'ENSG00000179743', 'ENSG00000148468', 'ENSG00000148110', 'ENSG00000039139', 'ENSG00000268400', 'ENSG00000116151', 'ENSG00000197620', 'ENSG00000154025', 'ENSG00000151690', 'ENSG00000255823', 'ENSG00000272425', 'ENSG00000188523', 'ENSG00000255517', 'ENSG00000196961', 'ENSG00000048740', 'ENSG00000225473', 'ENSG00000020256', 'ENSG00000106683', 'ENSG00000225233', 'ENSG00000233987', 'ENSG00000105204', 'ENSG00000136352', 'ENSG00000261754', 'ENSG00000183281', 'ENSG00000254377', 'ENSG00000272843', 'ENSG00000180596', 'ENSG00000138347', 'ENSG00000157335', 'ENSG00000227051', 'ENSG00000187624', 'ENSG00000231418', 'ENSG00000244462', 'ENSG00000250091', 'ENSG00000142207', 'ENSG00000164663', 'ENSG00000224655', 'ENSG00000003756', 'ENSG00000170523', 'ENSG00000141431', 'ENSG00000203279', 'ENSG00000268673', 'ENSG00000106404', 'ENSG00000254656', 'ENSG00000254438', 'ENSG00000255502', 'ENSG00000174348', 'ENSG00000251326', 'ENSG00000130827', 'ENSG00000145391', 'ENSG00000169499', 'ENSG00000250415', 'ENSG00000233349', 'ENSG00000130700', 'ENSG00000159128', 'ENSG00000232811', 'ENSG00000242715', 'ENSG00000140526', 'ENSG00000212747', 'ENSG00000254306', 'ENSG00000243795', 'ENSG00000232600', 'ENSG00000214827', 'ENSG00000234945', 'ENSG00000181982', 'ENSG00000223914', 'ENSG00000204351', 'ENSG00000271119', 'ENSG00000185261', 'ENSG00000151388', 'ENSG00000184345', 'ENSG00000223462', 'ENSG00000271434', 'ENSG00000172782', 'ENSG00000255462', 'ENSG00000005302', 'ENSG00000127515', 'ENSG00000261734', 'ENSG00000136938', 'ENSG00000169750', 'ENSG00000213588', 'ENSG00000229821', 'ENSG00000186862', 'ENSG00000151445', 'ENSG00000106609', 'ENSG00000120436', 'ENSG00000256551', 'ENSG00000180872', 'ENSG00000116977', 'ENSG00000100522', 'ENSG00000133706', 'ENSG00000248920', 'ENSG00000184436', 'ENSG00000233221', 'ENSG00000259209', 'ENSG00000125247', 'ENSG00000164621', 'ENSG00000104218', 'ENSG00000111834', 'ENSG00000234323', 'ENSG00000260286', 'ENSG00000228526', 'ENSG00000082068', 'ENSG00000205111', 'ENSG00000166261', 'ENSG00000197580', 'ENSG00000225819', 'ENSG00000168214', 'ENSG00000231373', 'ENSG00000255311', 'ENSG00000018625', 'ENSG00000236637', 'ENSG00000165006', 'ENSG00000113575', 'ENSG00000140396', 'ENSG00000225606', 'ENSG00000145337', 'ENSG00000135677', 'ENSG00000151379', 'ENSG00000229679', 'ENSG00000169155', 'ENSG00000254846', 'ENSG00000164761', 'ENSG00000234527', 'ENSG00000232465', 'ENSG00000149633', 'ENSG00000079974', 'ENSG00000225519', 'ENSG00000235303', 'ENSG00000229603', 'ENSG00000228587', 'ENSG00000108474', 'ENSG00000103061', 'ENSG00000253457', 'ENSG00000272370', 'ENSG00000120057', 'ENSG00000176230', 'ENSG00000246130', 'ENSG00000262903', 'ENSG00000043514', 'ENSG00000167483', 'ENSG00000145781', 'ENSG00000134775', 'ENSG00000086991', 'ENSG00000138107', 'ENSG00000255039', 'ENSG00000149150', 'ENSG00000003989', 'ENSG00000063438', 'ENSG00000175643', 'ENSG00000198062', 'ENSG00000183470', 'ENSG00000188766', 'ENSG00000270082', 'ENSG00000089094', 'ENSG00000263635', 'ENSG00000109814', 'ENSG00000173467', 'ENSG00000110066', 'ENSG00000125879', 'ENSG00000004838', 'ENSG00000227704', 'ENSG00000109113', 'ENSG00000247877', 'ENSG00000120594', 'ENSG00000108094', 'ENSG00000169016', 'ENSG00000125148', 'ENSG00000259181', 'ENSG00000257591', 'ENSG00000242689', 'ENSG00000141198', 'ENSG00000080709', 'ENSG00000142864', 'ENSG00000156675', 'ENSG00000157557', 'ENSG00000148215', 'ENSG00000230401', 'ENSG00000111247', 'ENSG00000227706', 'ENSG00000173715', 'ENSG00000205220', 'ENSG00000213799', 'ENSG00000173702', 'ENSG00000188729', 'ENSG00000099800', 'ENSG00000181555', 'ENSG00000234684', 'ENSG00000111481', 'ENSG00000232987', 'ENSG00000185630', 'ENSG00000136840', 'ENSG00000258444', 'ENSG00000164047', 'ENSG00000228661', 'ENSG00000139726', 'ENSG00000243477', 'ENSG00000259044', 'ENSG00000183401', 'ENSG00000168993', 'ENSG00000239839', 'ENSG00000233941', 'ENSG00000257435', 'ENSG00000179284', 'ENSG00000140992', 'ENSG00000135363', 'ENSG00000255420', 'ENSG00000115425', 'ENSG00000121380', 'ENSG00000225537', 'ENSG00000119041', 'ENSG00000123243', 'ENSG00000254863', 'ENSG00000139192', 'ENSG00000165632', 'ENSG00000230768', 'ENSG00000114054', 'ENSG00000049860', 'ENSG00000248698', 'ENSG00000213468', 'ENSG00000168591', 'ENSG00000132561', 'ENSG00000256286', 'ENSG00000139180', 'ENSG00000259456', 'ENSG00000255666', 'ENSG00000166987', 'ENSG00000167384', 'ENSG00000156140', 'ENSG00000119686', 'ENSG00000228567', 'ENSG00000237707', 'ENSG00000182810', 'ENSG00000234230', 'ENSG00000258858', 'ENSG00000256803', 'ENSG00000125144', 'ENSG00000126775', 'ENSG00000240770', 'ENSG00000205126', 'ENSG00000254120', 'ENSG00000172840', 'ENSG00000103381', 'ENSG00000178057', 'ENSG00000108064', 'ENSG00000120322', 'ENSG00000226996', 'ENSG00000214970', 'ENSG00000255663', 'ENSG00000227459', 'ENSG00000250948', 'ENSG00000139428', 'ENSG00000173473', 'ENSG00000231902', 'ENSG00000247903', 'ENSG00000229242', 'ENSG00000181458', 'ENSG00000101474', 'ENSG00000165209', 'ENSG00000046651', 'ENSG00000268061', 'ENSG00000221963', 'ENSG00000165325', 'ENSG00000100884', 'ENSG00000238189', 'ENSG00000234721', 'ENSG00000241962', 'ENSG00000148803', 'ENSG00000175206', 'ENSG00000248107', 'ENSG00000272277', 'ENSG00000120805', 'ENSG00000049249', 'ENSG00000090776', 'ENSG00000070731', 'ENSG00000148339', 'ENSG00000113194', 'ENSG00000110628', 'ENSG00000147873', 'ENSG00000164600', 'ENSG00000126705', 'ENSG00000235237', 'ENSG00000196502', 'ENSG00000154473', 'ENSG00000143921', 'ENSG00000105609', 'ENSG00000263924', 'ENSG00000184076', 'ENSG00000177822', 'ENSG00000261583', 'ENSG00000119283', 'ENSG00000235760', 'ENSG00000196361', 'ENSG00000109775', 'ENSG00000266369', 'ENSG00000179869', 'ENSG00000258285', 'ENSG00000123213', 'ENSG00000169836', 'ENSG00000184650', 'ENSG00000241151', 'ENSG00000138459', 'ENSG00000248576', 'ENSG00000235481', 'ENSG00000163734', 'ENSG00000106028', 'ENSG00000253298', 'ENSG00000259865', 'ENSG00000100652', 'ENSG00000251442', 'ENSG00000167491', 'ENSG00000258631', 'ENSG00000170748', 'ENSG00000166965', 'ENSG00000203661', 'ENSG00000108061', 'ENSG00000203857', 'ENSG00000131398', 'ENSG00000143632', 'ENSG00000258130', 'ENSG00000167914', 'ENSG00000221988', 'ENSG00000266728', 'ENSG00000246016', 'ENSG00000139330', 'ENSG00000101773', 'ENSG00000233718', 'ENSG00000204481', 'ENSG00000173918', 'ENSG00000268707', 'ENSG00000234186', 'ENSG00000233534', 'ENSG00000186451', 'ENSG00000114124', 'ENSG00000178741', 'ENSG00000249776', 'ENSG00000196407', 'ENSG00000228358', 'ENSG00000261396', 'ENSG00000119986', 'ENSG00000179038', 'ENSG00000161551', 'ENSG00000105698', 'ENSG00000164756', 'ENSG00000232640', 'ENSG00000205403', 'ENSG00000115091', 'ENSG00000173208', 'ENSG00000236874', 'ENSG00000197651', 'ENSG00000270403', 'ENSG00000203721', 'ENSG00000156253', 'ENSG00000231393', 'ENSG00000230133', 'ENSG00000179755', 'ENSG00000196341', 'ENSG00000131116', 'ENSG00000141696', 'ENSG00000081692', 'ENSG00000126368', 'ENSG00000088002', 'ENSG00000090266', 'ENSG00000162909', 'ENSG00000228763', 'ENSG00000186684', 'ENSG00000188038', 'ENSG00000254477', 'ENSG00000153446', 'ENSG00000251144', 'ENSG00000106771', 'ENSG00000078295', 'ENSG00000223675', 'ENSG00000229116', 'ENSG00000112053', 'ENSG00000117153', 'ENSG00000198843', 'ENSG00000164010', 'ENSG00000144120', 'ENSG00000187581', 'ENSG00000269300', 'ENSG00000070770', 'ENSG00000148341', 'ENSG00000261798', 'ENSG00000230149', 'ENSG00000146360', 'ENSG00000137936', 'ENSG00000123338', 'ENSG00000120733', 'ENSG00000258902', 'ENSG00000139154', 'ENSG00000268654', 'ENSG00000254415', 'ENSG00000260907', 'ENSG00000254043', 'ENSG00000243636', 'ENSG00000198755', 'ENSG00000101435', 'ENSG00000118369', 'ENSG00000189067', 'ENSG00000267761', 'ENSG00000118004', 'ENSG00000160808', 'ENSG00000178988', 'ENSG00000179144', 'ENSG00000184814', 'ENSG00000171634', 'ENSG00000136682', 'ENSG00000114544', 'ENSG00000245888', 'ENSG00000259776', 'ENSG00000115008', 'ENSG00000153246', 'ENSG00000269858', 'ENSG00000237357', 'ENSG00000159212', 'ENSG00000249153', 'ENSG00000214842', 'ENSG00000134757', 'ENSG00000131849', 'ENSG00000157087', 'ENSG00000236427', 'ENSG00000250555', 'ENSG00000268658', 'ENSG00000205129', 'ENSG00000212856', 'ENSG00000164002', 'ENSG00000273133', 'ENSG00000147099', 'ENSG00000100321', 'ENSG00000163528', 'ENSG00000215403', 'ENSG00000165113', 'ENSG00000137522', 'ENSG00000125637', 'ENSG00000215644', 'ENSG00000181036', 'ENSG00000175274', 'ENSG00000205544', 'ENSG00000258600', 'ENSG00000233975', 'ENSG00000260234', 'ENSG00000261618', 'ENSG00000236651', 'ENSG00000233818', 'ENSG00000268628', 'ENSG00000108846', 'ENSG00000235268', 'ENSG00000143502', 'ENSG00000126709', 'ENSG00000259077', 'ENSG00000231297', 'ENSG00000170289', 'ENSG00000234474', 'ENSG00000134215', 'ENSG00000240972', 'ENSG00000125730', 'ENSG00000204961', 'ENSG00000266844', 'ENSG00000183783', 'ENSG00000253783', 'ENSG00000256862', 'ENSG00000172243', 'ENSG00000230831', 'ENSG00000196787', 'ENSG00000085449', 'ENSG00000186020', 'ENSG00000198185', 'ENSG00000153015', 'ENSG00000248330', 'ENSG00000260192', 'ENSG00000228658', 'ENSG00000137752', 'ENSG00000232401', 'ENSG00000233701', 'ENSG00000181104', 'ENSG00000215067', 'ENSG00000160050', 'ENSG00000089195', 'ENSG00000006576', 'ENSG00000073536', 'ENSG00000107874', 'ENSG00000076043', 'ENSG00000163527', 'ENSG00000245059', 'ENSG00000245281', 'ENSG00000121310', 'ENSG00000186081', 'ENSG00000247130', 'ENSG00000035115', 'ENSG00000245146', 'ENSG00000135241', 'ENSG00000124356', 'ENSG00000271996', 'ENSG00000175322', 'ENSG00000260913', 'ENSG00000100095', 'ENSG00000101391', 'ENSG00000064703', 'ENSG00000260874', 'ENSG00000144451', 'ENSG00000131471', 'ENSG00000102103', 'ENSG00000099381', 'ENSG00000230322', 'ENSG00000144214', 'ENSG00000253404', 'ENSG00000197885', 'ENSG00000108384', 'ENSG00000151208', 'ENSG00000204764', 'ENSG00000249641', 'ENSG00000186318', 'ENSG00000262049', 'ENSG00000151694', 'ENSG00000259376', 'ENSG00000140807', 'ENSG00000229639', 'ENSG00000230597', 'ENSG00000268926', 'ENSG00000115694', 'ENSG00000273367', 'ENSG00000184445', 'ENSG00000184009', 'ENSG00000239440', 'ENSG00000007944', 'ENSG00000256672', 'ENSG00000246422', 'ENSG00000054654', 'ENSG00000226953', 'ENSG00000271141', 'ENSG00000085998', 'ENSG00000164252', 'ENSG00000255858', 'ENSG00000223528', 'ENSG00000174197', 'ENSG00000229201', 'ENSG00000205726', 'ENSG00000168477', 'ENSG00000167257', 'ENSG00000160993', 'ENSG00000238194', 'ENSG00000263860', 'ENSG00000261154', 'ENSG00000177202', 'ENSG00000115526', 'ENSG00000125337', 'ENSG00000259840', 'ENSG00000205022', 'ENSG00000168612', 'ENSG00000111252', 'ENSG00000172081', 'ENSG00000241131', 'ENSG00000272710', 'ENSG00000257398', 'ENSG00000170627', 'ENSG00000235412', 'ENSG00000100938', 'ENSG00000158639', 'ENSG00000232680', 'ENSG00000122565', 'ENSG00000256077', 'ENSG00000197496', 'ENSG00000205790', 'ENSG00000251575', 'ENSG00000241345', 'ENSG00000233358', 'ENSG00000126883', 'ENSG00000163235', 'ENSG00000257438', 'ENSG00000151090', 'ENSG00000236371', 'ENSG00000141837', 'ENSG00000235385', 'ENSG00000167104', 'ENSG00000267555', 'ENSG00000076003', 'ENSG00000236053', 'ENSG00000269931', 'ENSG00000249941', 'ENSG00000198785', 'ENSG00000119614', 'ENSG00000198954', 'ENSG00000270210', 'ENSG00000262703', 'ENSG00000205930', 'ENSG00000273372', 'ENSG00000159556', 'ENSG00000262791', 'ENSG00000258404', 'ENSG00000231143', 'ENSG00000250021', 'ENSG00000103319', 'ENSG00000254298', 'ENSG00000139132', 'ENSG00000224122', 'ENSG00000250431', 'ENSG00000260937', 'ENSG00000107404', 'ENSG00000225386', 'ENSG00000124091', 'ENSG00000151224', 'ENSG00000265399', 'ENSG00000120694', 'ENSG00000248425', 'ENSG00000234438', 'ENSG00000233070', 'ENSG00000156804', 'ENSG00000064270', 'ENSG00000256789', 'ENSG00000100934', 'ENSG00000271218', 'ENSG00000117984', 'ENSG00000176909', 'ENSG00000154143', 'ENSG00000248405', 'ENSG00000126261', 'ENSG00000234602', 'ENSG00000121067', 'ENSG00000167757', 'ENSG00000228573', 'ENSG00000162373', 'ENSG00000115282', 'ENSG00000112144', 'ENSG00000260848', 'ENSG00000248774', 'ENSG00000223438', 'ENSG00000154162', 'ENSG00000136240', 'ENSG00000124253', 'ENSG00000263531', 'ENSG00000270947', 'ENSG00000177614', 'ENSG00000151135', 'ENSG00000203321', 'ENSG00000161914', 'ENSG00000172020', 'ENSG00000243179', 'ENSG00000269371', 'ENSG00000140319', 'ENSG00000178055', 'ENSG00000260473', 'ENSG00000182111', 'ENSG00000266977', 'ENSG00000155066', 'ENSG00000152804', 'ENSG00000267109', 'ENSG00000157703', 'ENSG00000233421', 'ENSG00000110047', 'ENSG00000094963', 'ENSG00000163785', 'ENSG00000100151', 'ENSG00000227475', 'ENSG00000197177', 'ENSG00000180776', 'ENSG00000106144', 'ENSG00000176904', 'ENSG00000184302', 'ENSG00000204464', 'ENSG00000111817', 'ENSG00000176742', 'ENSG00000103021', 'ENSG00000238057', 'ENSG00000258694', 'ENSG00000154328', 'ENSG00000261485', 'ENSG00000230453', 'ENSG00000231500', 'ENSG00000136758', 'ENSG00000005075', 'ENSG00000213533', 'ENSG00000261449', 'ENSG00000267646', 'ENSG00000261832', 'ENSG00000245748', 'ENSG00000228730', 'ENSG00000196704', 'ENSG00000204301', 'ENSG00000177485', 'ENSG00000002822', 'ENSG00000204323', 'ENSG00000115274', 'ENSG00000106560', 'ENSG00000261043', 'ENSG00000174957', 'ENSG00000264449', 'ENSG00000224186', 'ENSG00000267092', 'ENSG00000100908', 'ENSG00000187098', 'ENSG00000248694', 'ENSG00000256683', 'ENSG00000104907', 'ENSG00000115561', 'ENSG00000175115', 'ENSG00000125753', 'ENSG00000256769', 'ENSG00000228889', 'ENSG00000122787', 'ENSG00000143612', 'ENSG00000227481', 'ENSG00000010278', 'ENSG00000166130', 'ENSG00000261498', 'ENSG00000261213', 'ENSG00000171867', 'ENSG00000237735', 'ENSG00000091483', 'ENSG00000084207', 'ENSG00000188266', 'ENSG00000268804', 'ENSG00000204138', 'ENSG00000175514', 'ENSG00000262877', 'ENSG00000125629', 'ENSG00000129824', 'ENSG00000183617', 'ENSG00000111653', 'ENSG00000196366', 'ENSG00000223944', 'ENSG00000233340', 'ENSG00000071859', 'ENSG00000133124', 'ENSG00000234308', 'ENSG00000187838', 'ENSG00000147799', 'ENSG00000109991', 'ENSG00000242516', 'ENSG00000136156', 'ENSG00000143147', 'ENSG00000240973', 'ENSG00000223501', 'ENSG00000173200', 'ENSG00000235192', 'ENSG00000222020', 'ENSG00000106415', 'ENSG00000264149', 'ENSG00000142606', 'ENSG00000242120', 'ENSG00000165669', 'ENSG00000237346', 'ENSG00000112232', 'ENSG00000175354', 'ENSG00000139151', 'ENSG00000135093', 'ENSG00000188092', 'ENSG00000258511', 'ENSG00000135912', 'ENSG00000114166', 'ENSG00000256923', 'ENSG00000261235', 'ENSG00000228809', 'ENSG00000258301', 'ENSG00000170801', 'ENSG00000221882', 'ENSG00000135334', 'ENSG00000152503', 'ENSG00000260504', 'ENSG00000258517', 'ENSG00000178412', 'ENSG00000183741', 'ENSG00000171466', 'ENSG00000267387', 'ENSG00000255974', 'ENSG00000117419', 'ENSG00000132321', 'ENSG00000182022', 'ENSG00000224405', 'ENSG00000104888', 'ENSG00000179397', 'ENSG00000105707', 'ENSG00000165914', 'ENSG00000170921', 'ENSG00000049089', 'ENSG00000235705', 'ENSG00000108786', 'ENSG00000155906', 'ENSG00000255910', 'ENSG00000232225', 'ENSG00000185985', 'ENSG00000134802', 'ENSG00000273238', 'ENSG00000257259', 'ENSG00000169418', 'ENSG00000163281', 'ENSG00000239921', 'ENSG00000197586', 'ENSG00000204435', 'ENSG00000196166', 'ENSG00000177462', 'ENSG00000254171', 'ENSG00000248397', 'ENSG00000171488', 'ENSG00000179407', 'ENSG00000263382', 'ENSG00000120341', 'ENSG00000100811', 'ENSG00000239713', 'ENSG00000261482', 'ENSG00000253686', 'ENSG00000105486', 'ENSG00000163312', 'ENSG00000101639', 'ENSG00000162073', 'ENSG00000236046', 'ENSG00000272478', 'ENSG00000142539', 'ENSG00000246731', 'ENSG00000163389', 'ENSG00000242593', 'ENSG00000171942', 'ENSG00000234699', 'ENSG00000236822', 'ENSG00000225337', 'ENSG00000089639', 'ENSG00000166016', 'ENSG00000232259', 'ENSG00000109861', 'ENSG00000229278', 'ENSG00000141562', 'ENSG00000224559', 'ENSG00000124237', 'ENSG00000084444', 'ENSG00000228675', 'ENSG00000092295', 'ENSG00000235645', 'ENSG00000155111', 'ENSG00000235641', 'ENSG00000243709', 'ENSG00000139329', 'ENSG00000091583', 'ENSG00000154319', 'ENSG00000205174', 'ENSG00000258938', 'ENSG00000268390', 'ENSG00000085265', 'ENSG00000171824', 'ENSG00000261090', 'ENSG00000230090', 'ENSG00000213057', 'ENSG00000269504', 'ENSG00000178591', 'ENSG00000157191', 'ENSG00000249348', 'ENSG00000188676', 'ENSG00000232307', 'ENSG00000155511', 'ENSG00000164334', 'ENSG00000198039', 'ENSG00000042304', 'ENSG00000259540', 'ENSG00000172264', 'ENSG00000173976', 'ENSG00000163374', 'ENSG00000134321', 'ENSG00000188162', 'ENSG00000246100', 'ENSG00000104818', 'ENSG00000234730', 'ENSG00000157985', 'ENSG00000258313', 'ENSG00000178694', 'ENSG00000112305', 'ENSG00000250632', 'ENSG00000159579', 'ENSG00000224814', 'ENSG00000164920', 'ENSG00000249831', 'ENSG00000258573', 'ENSG00000214706', 'ENSG00000144749', 'ENSG00000145220', 'ENSG00000067048', 'ENSG00000227356', 'ENSG00000270149', 'ENSG00000254204', 'ENSG00000130758', 'ENSG00000165516', 'ENSG00000131873', 'ENSG00000272715', 'ENSG00000125863', 'ENSG00000010932', 'ENSG00000131462', 'ENSG00000224500', 'ENSG00000008083', 'ENSG00000157219', 'ENSG00000224459', 'ENSG00000125746', 'ENSG00000116809', 'ENSG00000106927', 'ENSG00000168904', 'ENSG00000245662', 'ENSG00000112599', 'ENSG00000097021', 'ENSG00000068305', 'ENSG00000250349', 'ENSG00000147100', 'ENSG00000197046', 'ENSG00000231050', 'ENSG00000175646', 'ENSG00000125878', 'ENSG00000261035', 'ENSG00000226101', 'ENSG00000197283', 'ENSG00000235699', 'ENSG00000184967', 'ENSG00000227695', 'ENSG00000248771', 'ENSG00000176194', 'ENSG00000110046', 'ENSG00000188582', 'ENSG00000253873', 'ENSG00000162755', 'ENSG00000161652', 'ENSG00000230826', 'ENSG00000091010', 'ENSG00000196944', 'ENSG00000248468', 'ENSG00000155970', 'ENSG00000122679', 'ENSG00000106302', 'ENSG00000134595', 'ENSG00000110583', 'ENSG00000271755', 'ENSG00000170889', 'ENSG00000235564', 'ENSG00000232192', 'ENSG00000095321', 'ENSG00000260733', 'ENSG00000156471', 'ENSG00000236437', 'ENSG00000233384', 'ENSG00000234956', 'ENSG00000135406', 'ENSG00000260223', 'ENSG00000164619', 'ENSG00000268996', 'ENSG00000267722', 'ENSG00000189350', 'ENSG00000183251', 'ENSG00000058272', 'ENSG00000234832', 'ENSG00000260693', 'ENSG00000262294', 'ENSG00000177150', 'ENSG00000106635', 'ENSG00000109424', 'ENSG00000133943', 'ENSG00000081051', 'ENSG00000147234', 'ENSG00000132274', 'ENSG00000258710', 'ENSG00000224943', 'ENSG00000251173', 'ENSG00000125388', 'ENSG00000225084', 'ENSG00000225234', 'ENSG00000232746', 'ENSG00000119509', 'ENSG00000235288', 'ENSG00000006283', 'ENSG00000250317', 'ENSG00000234056', 'ENSG00000237166', 'ENSG00000229775', 'ENSG00000272933', 'ENSG00000232290', 'ENSG00000261653', 'ENSG00000188000', 'ENSG00000259724', 'ENSG00000109099', 'ENSG00000182185', 'ENSG00000167601', 'ENSG00000109670', 'ENSG00000249459', 'ENSG00000269506', 'ENSG00000137404', 'ENSG00000268560', 'ENSG00000162630', 'ENSG00000117758', 'ENSG00000255655', 'ENSG00000105287', 'ENSG00000134909', 'ENSG00000175806', 'ENSG00000158201', 'ENSG00000001460', 'ENSG00000203362', 'ENSG00000185477', 'ENSG00000187189', 'ENSG00000258084', 'ENSG00000101310', 'ENSG00000163762', 'ENSG00000268461', 'ENSG00000136457', 'ENSG00000065518', 'ENSG00000141391', 'ENSG00000258525', 'ENSG00000172466', 'ENSG00000128656', 'ENSG00000155966', 'ENSG00000151704', 'ENSG00000157796', 'ENSG00000250193', 'ENSG00000260740', 'ENSG00000163406', 'ENSG00000241792', 'ENSG00000120833', 'ENSG00000105928', 'ENSG00000197745', 'ENSG00000270019', 'ENSG00000111877', 'ENSG00000071282', 'ENSG00000232807', 'ENSG00000249513', 'ENSG00000263388', 'ENSG00000237262', 'ENSG00000232993', 'ENSG00000234707', 'ENSG00000234478', 'ENSG00000253164', 'ENSG00000256281', 'ENSG00000223911', 'ENSG00000110921', 'ENSG00000259985', 'ENSG00000227659', 'ENSG00000134258', 'ENSG00000146776', 'ENSG00000137714', 'ENSG00000169906', 'ENSG00000155324', 'ENSG00000072518', 'ENSG00000237489', 'ENSG00000259760', 'ENSG00000241211', 'ENSG00000174483', 'ENSG00000269483', 'ENSG00000226375', 'ENSG00000227150', 'ENSG00000115355', 'ENSG00000124145', 'ENSG00000131143', 'ENSG00000265218', 'ENSG00000078814', 'ENSG00000128185', 'ENSG00000215529', 'ENSG00000125848', 'ENSG00000227227', 'ENSG00000143033', 'ENSG00000100221', 'ENSG00000224028', 'ENSG00000137413', 'ENSG00000179262', 'ENSG00000236373', 'ENSG00000227045', 'ENSG00000169126', 'ENSG00000179271', 'ENSG00000177476', 'ENSG00000181513', 'ENSG00000265205', 'ENSG00000153157', 'ENSG00000266120', 'ENSG00000125868', 'ENSG00000146674', 'ENSG00000242242', 'ENSG00000103507', 'ENSG00000181722', 'ENSG00000132026', 'ENSG00000125046', 'ENSG00000273341', 'ENSG00000232995', 'ENSG00000171606', 'ENSG00000166800', 'ENSG00000106992', 'ENSG00000251584', 'ENSG00000258660', 'ENSG00000259336', 'ENSG00000235533', 'ENSG00000234275', 'ENSG00000136720', 'ENSG00000143595', 'ENSG00000248783', 'ENSG00000231492', 'ENSG00000249592', 'ENSG00000180233', 'ENSG00000101463', 'ENSG00000035403', 'ENSG00000081913', 'ENSG00000254972', 'ENSG00000273240', 'ENSG00000273265', 'ENSG00000205250', 'ENSG00000180815', 'ENSG00000139287', 'ENSG00000138594', 'ENSG00000224051', 'ENSG00000127564', 'ENSG00000198829', 'ENSG00000180354', 'ENSG00000196589', 'ENSG00000250340', 'ENSG00000255804', 'ENSG00000001167', 'ENSG00000227155', 'ENSG00000135632', 'ENSG00000128989', 'ENSG00000130997', 'ENSG00000188909', 'ENSG00000250748', 'ENSG00000251320', 'ENSG00000256948', 'ENSG00000206113', 'ENSG00000267097', 'ENSG00000259395', 'ENSG00000227488', 'ENSG00000172785', 'ENSG00000268472', 'ENSG00000121068', 'ENSG00000255351', 'ENSG00000031081', 'ENSG00000107938', 'ENSG00000255432', 'ENSG00000120533', 'ENSG00000197694', 'ENSG00000167797', 'ENSG00000127252', 'ENSG00000227619', 'ENSG00000203909', 'ENSG00000270661', 'ENSG00000142534', 'ENSG00000236009', 'ENSG00000204516', 'ENSG00000215568', 'ENSG00000162413', 'ENSG00000178734', 'ENSG00000255843', 'ENSG00000184557', 'ENSG00000100418', 'ENSG00000180353', 'ENSG00000106266', 'ENSG00000105270', 'ENSG00000177885', 'ENSG00000164045', 'ENSG00000167588', 'ENSG00000263986', 'ENSG00000188906', 'ENSG00000238043', 'ENSG00000143032', 'ENSG00000257940', 'ENSG00000255814', 'ENSG00000135914', 'ENSG00000189280', 'ENSG00000154016', 'ENSG00000249746', 'ENSG00000104983', 'ENSG00000096433', 'ENSG00000172404', 'ENSG00000138756', 'ENSG00000126254', 'ENSG00000162885', 'ENSG00000270011', 'ENSG00000237250', 'ENSG00000272772', 'ENSG00000205846', 'ENSG00000260903', 'ENSG00000180822', 'ENSG00000267582', 'ENSG00000078795', 'ENSG00000172273', 'ENSG00000196227', 'ENSG00000258839', 'ENSG00000179213', 'ENSG00000114573', 'ENSG00000248443', 'ENSG00000258903', 'ENSG00000008196', 'ENSG00000183734', 'ENSG00000168907', 'ENSG00000226320', 'ENSG00000272564', 'ENSG00000089041', 'ENSG00000212657', 'ENSG00000169154', 'ENSG00000175329', 'ENSG00000165837', 'ENSG00000137252', 'ENSG00000100721', 'ENSG00000239886', 'ENSG00000236687', 'ENSG00000268001', 'ENSG00000254205', 'ENSG00000270118', 'ENSG00000271204', 'ENSG00000162383', 'ENSG00000254820', 'ENSG00000214013', 'ENSG00000261313', 'ENSG00000262950', 'ENSG00000172380', 'ENSG00000260957', 'ENSG00000267112', 'ENSG00000144668', 'ENSG00000223547', 'ENSG00000138071', 'ENSG00000231791', 'ENSG00000073417', 'ENSG00000250929', 'ENSG00000174255', 'ENSG00000197016', 'ENSG00000228778', 'ENSG00000172115', 'ENSG00000122711', 'ENSG00000196420', 'ENSG00000102243', 'ENSG00000075151', 'ENSG00000198001', 'ENSG00000186583', 'ENSG00000165626', 'ENSG00000233610', 'ENSG00000132950', 'ENSG00000143061', 'ENSG00000164830', 'ENSG00000263098', 'ENSG00000143622', 'ENSG00000111885', 'ENSG00000107317', 'ENSG00000250133', 'ENSG00000231310', 'ENSG00000156006', 'ENSG00000140988', 'ENSG00000175018', 'ENSG00000058085', 'ENSG00000174914', 'ENSG00000105472', 'ENSG00000142149', 'ENSG00000235419', 'ENSG00000183067', 'ENSG00000004975', 'ENSG00000223834', 'ENSG00000250041', 'ENSG00000257997', 'ENSG00000205858', 'ENSG00000164885', 'ENSG00000186448', 'ENSG00000186493', 'ENSG00000255958', 'ENSG00000248799', 'ENSG00000204379', 'ENSG00000226130', 'ENSG00000251497', 'ENSG00000144227', 'ENSG00000168876', 'ENSG00000142910', 'ENSG00000225407', 'ENSG00000262074', 'ENSG00000159184', 'ENSG00000113648', 'ENSG00000223882', 'ENSG00000198454', 'ENSG00000175482', 'ENSG00000254535', 'ENSG00000254756', 'ENSG00000257671', 'ENSG00000240573', 'ENSG00000057019', 'ENSG00000204475', 'ENSG00000249261', 'ENSG00000186395', 'ENSG00000184481', 'ENSG00000076356', 'ENSG00000101981', 'ENSG00000157978', 'ENSG00000231083', 'ENSG00000177335', 'ENSG00000228539', 'ENSG00000205078', 'ENSG00000237529', 'ENSG00000224418', 'ENSG00000122877', 'ENSG00000237232', 'ENSG00000267016', 'ENSG00000204444', 'ENSG00000265995', 'ENSG00000257341', 'ENSG00000248079', 'ENSG00000154760', 'ENSG00000249593', 'ENSG00000146477', 'ENSG00000003137', 'ENSG00000164128', 'ENSG00000228697', 'ENSG00000269476', 'ENSG00000253206', 'ENSG00000000003', 'ENSG00000149781', 'ENSG00000198400', 'ENSG00000260271', 'ENSG00000124003', 'ENSG00000175591', 'ENSG00000256637', 'ENSG00000179165', 'ENSG00000272669', 'ENSG00000174827', 'ENSG00000259070', 'ENSG00000125910', 'ENSG00000055332', 'ENSG00000105669', 'ENSG00000267695', 'ENSG00000256343', 'ENSG00000115461', 'ENSG00000120008', 'ENSG00000105205', 'ENSG00000143867', 'ENSG00000259616', 'ENSG00000249160', 'ENSG00000147439', 'ENSG00000229012', 'ENSG00000135175', 'ENSG00000181718', 'ENSG00000184905', 'ENSG00000126233', 'ENSG00000258701', 'ENSG00000198301', 'ENSG00000108379', 'ENSG00000150667', 'ENSG00000163995', 'ENSG00000079999', 'ENSG00000149743', 'ENSG00000251200', 'ENSG00000166961', 'ENSG00000221923', 'ENSG00000183624', 'ENSG00000271590', 'ENSG00000257634', 'ENSG00000162402', 'ENSG00000228634', 'ENSG00000163636', 'ENSG00000110717', 'ENSG00000168843', 'ENSG00000254349', 'ENSG00000138395', 'ENSG00000085415', 'ENSG00000261636', 'ENSG00000126391', 'ENSG00000169684', 'ENSG00000235010', 'ENSG00000185896', 'ENSG00000181027', 'ENSG00000000971', 'ENSG00000091106', 'ENSG00000254605', 'ENSG00000140675', 'ENSG00000196074', 'ENSG00000105971', 'ENSG00000225255', 'ENSG00000118985', 'ENSG00000172828', 'ENSG00000054277', 'ENSG00000226115', 'ENSG00000214681', 'ENSG00000214184', 'ENSG00000250334', 'ENSG00000139343', 'ENSG00000162552', 'ENSG00000164185', 'ENSG00000107882', 'ENSG00000251655', 'ENSG00000136535', 'ENSG00000087269', 'ENSG00000260739', 'ENSG00000257056', 'ENSG00000267374', 'ENSG00000182117', 'ENSG00000214941', 'ENSG00000134153', 'ENSG00000262681', 'ENSG00000251015', 'ENSG00000171916', 'ENSG00000177398', 'ENSG00000253888', 'ENSG00000205899', 'ENSG00000167397', 'ENSG00000166685', 'ENSG00000248866', 'ENSG00000231248', 'ENSG00000075399', 'ENSG00000179083', 'ENSG00000117262', 'ENSG00000119912', 'ENSG00000101888', 'ENSG00000243207', 'ENSG00000261751', 'ENSG00000238121', 'ENSG00000139874', 'ENSG00000272402', 'ENSG00000241769', 'ENSG00000253524', 'ENSG00000250106', 'ENSG00000254295', 'ENSG00000234394', 'ENSG00000268006', 'ENSG00000159792', 'ENSG00000157510', 'ENSG00000267633', 'ENSG00000228369', 'ENSG00000171811', 'ENSG00000164953', 'ENSG00000143786', 'ENSG00000238198', 'ENSG00000239791', 'ENSG00000223504', 'ENSG00000250511', 'ENSG00000203667', 'ENSG00000242659', 'ENSG00000164611', 'ENSG00000081307', 'ENSG00000152457', 'ENSG00000163319', 'ENSG00000232935', 'ENSG00000223764', 'ENSG00000183638', 'ENSG00000145425', 'ENSG00000179044']
      → save terms via .add_validated_from_var_index()

• mapping depmap_id on CellLine.ontology_id

!    found 183 validated terms: ['ACH-000390', 'ACH-000444', 'ACH-000396', 'ACH-000997', 'ACH-000723', 'ACH-000504', 'ACH-001190', 'ACH-000834', 'ACH-000880', 'ACH-000717', 'ACH-000824', 'ACH-000713', 'ACH-000219', 'ACH-000762', 'ACH-000022', 'ACH-000750', 'ACH-000603', 'ACH-000228', 'ACH-000423', 'ACH-000553', 'ACH-000001', 'ACH-000270', 'ACH-000374', 'ACH-000649', 'ACH-000174', 'ACH-000791', 'ACH-000764', 'ACH-000873', 'ACH-000510', 'ACH-000376', 'ACH-000211', 'ACH-000875', 'ACH-000749', 'ACH-000347', 'ACH-000416', 'ACH-000565', 'ACH-000463', 'ACH-000397', 'ACH-000200', 'ACH-000086', 'ACH-000537', 'ACH-000479', 'ACH-000666', 'ACH-000897', 'ACH-000927', 'ACH-000415', 'ACH-000900', 'ACH-000189', 'ACH-000982', 'ACH-000288', 'ACH-000244', 'ACH-000323', 'ACH-000916', 'ACH-000704', 'ACH-000488', 'ACH-000235', 'ACH-000142', 'ACH-000685', 'ACH-000021', 'ACH-000159', 'ACH-000884', 'ACH-001307', 'ACH-000265', 'ACH-000756', 'ACH-000047', 'ACH-000252', 'ACH-000471', 'ACH-000977', 'ACH-000888', 'ACH-000209', 'ACH-000502', 'ACH-000212', 'ACH-000652', 'ACH-000367', 'ACH-000434', 'ACH-000950', 'ACH-000549', 'ACH-000947', 'ACH-000833', 'ACH-000452', 'ACH-000255', 'ACH-000822', 'ACH-000670', 'ACH-000896', 'ACH-000589', 'ACH-000886', 'ACH-000018', 'ACH-000672', 'ACH-000428', 'ACH-000911', 'ACH-000274', 'ACH-000785', 'ACH-000868', 'ACH-000866', 'ACH-000458', 'ACH-000882', 'ACH-000954', 'ACH-000958', 'ACH-000738', 'ACH-000734', 'ACH-000849', 'ACH-000973', 'ACH-000961', 'ACH-000098', 'ACH-000486', 'ACH-000605', 'ACH-000837', 'ACH-000460', 'ACH-000368', 'ACH-000123', 'ACH-000329', 'ACH-000186', 'ACH-000939', 'ACH-000657', 'ACH-000622', 'ACH-000015', 'ACH-000956', 'ACH-000014', 'ACH-000037', 'ACH-000966', 'ACH-000335', 'ACH-000579', 'ACH-000769', 'ACH-000644', 'ACH-000445', 'ACH-000701', 'ACH-000545', 'ACH-000595', 'ACH-000149', 'ACH-000178', 'ACH-000164', 'ACH-000023', 'ACH-000292', 'ACH-000517', 'ACH-000117', 'ACH-000527', 'ACH-000572', 'ACH-000048', 'ACH-000967', 'ACH-000324', 'ACH-000601', 'ACH-000680', 'ACH-000026', 'ACH-000411', 'ACH-000569', 'ACH-000669', 'ACH-000277', 'ACH-000941', 'ACH-000495', 'ACH-000096', 'ACH-000085', 'ACH-000268', 'ACH-000302', 'ACH-000393', 'ACH-000618', 'ACH-000624', 'ACH-000774', 'ACH-000552', 'ACH-000407', 'ACH-000936', 'ACH-000681', 'ACH-000971', 'ACH-000237', 'ACH-000976', 'ACH-000776', 'ACH-000650', 'ACH-000803', 'ACH-000535', 'ACH-000341', 'ACH-000090', 'ACH-000662', 'ACH-000748', 'ACH-000661', 'ACH-001239', 'ACH-000826', 'ACH-000035', 'ACH-000343', 'ACH-000847', 'ACH-000163', 'ACH-000906', 'ACH-000903', 'ACH-000449', 'ACH-000842']
      → save terms via .add_validated_from('depmap_id')

• mapping cell_line on CellLine.name

!    found 30 validated terms: ['YD-10B', 'UM-UC-1', 'YD-38', 'LOX IMVI', 'SH-10-TC', 'SF-295', 'SNU-761', 'KNS-81', 'BICR 6', 'L3.3', 'SNU-1079', 'TCC-PAN2', 'SNU-1076', 'UO-31', 'HCC-1195', 'JHH-5', 'SNU-8', 'SNU-1105', 'COV434', 'IGROV1', 'KNS-60', 'RMUG-S', 'SNU-410', '253J-BV', 'SNU-245', 'SNU-1077', 'SNU-1041', 'RERF-LC-Ad2', 'SNU-685', 'WM1799']
      → save terms via .add_validated_from('cell_line')

!    153 terms are not validated: 'LUDLU-1 cell', 'RCB1900 cell', 'J82 cell', 'HCT-15 cell', 'SNB75 cell', 'SK-MEL-2 cell', 'AGS cell', 'COLO-680N cell', 'KYSE-510 cell', 'Caov-3 cell', 'A-375 cell', 'PA-TU-8988S cell', 'BEN cell', 'BICR 31 cell', 'SK-MEL-3 cell', 'RCB1905 cell', 'NIH:OVCAR-3 cell', 'HPAC cell', 'HCC1143 cell', '786-O cell', ...
      → fix typos, remove non-existent values, or save terms via .add_new_from('cell_line')

• mapping disease on Disease.name

!    found 21 validated terms: ['lung cancer', 'urinary bladder carcinoma', 'colorectal cancer', 'head and neck cancer', 'brain cancer', 'skin cancer', 'gastric cancer', 'esophageal cancer', 'ovarian cancer', 'malignant pancreatic neoplasm', 'breast cancer', 'kidney cancer', 'thyroid cancer', 'uterine corpus cancer', 'liver cancer', 'prostate cancer', 'bile duct cancer', 'sarcoma', 'neuroblastoma', 'rhabdoid tumor', 'bone cancer']
      → save terms via .add_validated_from('disease')

✓ organism is validated against Organism.name

• mapping perturbation_type on ULabel.name

!    2 terms are not validated: 'drug', 'CRISPR'
      → fix typos, remove non-existent values, or save terms via .add_new_from('perturbation_type')

• mapping sex on Phenotype.name

!    3 terms are not validated: 'Male', 'Female', 'Unknown'
      → fix typos, remove non-existent values, or save terms via .add_new_from('sex')

• mapping time on ULabel.name

!    4 terms are not validated: '24', '72, 96', '3, 6, 12, 24, 48', '6'
      → fix typos, remove non-existent values, or save terms via .add_new_from('time')

• mapping tissue_type on ULabel.name

!    1 terms is not validated: 'cell_line'
      → fix typos, remove non-existent values, or save terms via .add_new_from('tissue_type')

False

curate.add_validated_from_var_index()
curate.add_validated_from("depmap_id")
curate.add_new_from("perturbation_type")
curate.add_new_from("sex")
curate.add_new_from("time")
curate.add_new_from("tissue_type")
curate.add_validated_from("disease")
curate.add_new_from("cell_line")

Modeling and curating perturbation metadata¶

The dataset has two types of perturbations: CRISPR and Compounds. We will create their records and associated targets separately.

crispr_metadata = adata.obs[adata.obs["perturbation_type"] == "CRISPR"]
drug_metadata = adata.obs[adata.obs["perturbation_type"] == "drug"]

The wetlab schema has two major components:

wetlab.EnvironmentalTreatment to model perturbations such as heat, wetlab.GeneticTreatment to model perturbations such as CRISPR, and wetlab.CompoundTreatment to model, for example, drugs. Several treatments together can be modeled using wetlab.CombinationTreatment.
Known targets of treatments can be modeled through wetlab.TreatmentTarget which can be one or several of bionty.Gene, bionty.Protein, or bionty.Pathway records.

Genetic perturbations¶

Genetic perturbations can be modeled in two ways depending on the available information by populating a:

wetlab.GeneticTreatment record if the system such as the guide RNA name or sequence, the on- and off-target scores are known.
wetlab.TreatmentTarget record that links to bionty.Gene records.

crispr_metadata.head(3)

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	depmap_id	cancer	cell_det_rate	cell_line	cell_quality	channel	disease	dose_unit	dose_value	doublet_CL1	doublet_CL2	doublet_GMM_prob	doublet_dev_imp	doublet_z_margin	hash_assignment	hash_tag	num_SNPs	organism	perturbation	perturbation_type	sex	singlet_ID	singlet_dev	singlet_dev_z	singlet_margin	singlet_z_margin	time	tissue_type	tot_reads	nperts	ngenes	ncounts	percent_mito	percent_ribo	chembl-ID
TAGTTGGAGATCGATA	ACH-000723	True	0.132708	YD-10B	low_quality	nan	head and neck cancer	nan	NaN	YD10B_UPPER_AERODIGESTIVE_TRACT	647V_URINARY_TRACT	NaN	0.156492	1.556214	nan	nan	874	human	sggpx4-2	CRISPR	Male	YD10B_UPPER_AERODIGESTIVE_TRACT	0.292802	3.272682	0.016459	0.330120	72, 96	cell_line	2105	1	4341	20693.0	0.695887	16.242208	NaN
CATCGGGGTTCATGGT	ACH-000219	True	0.087860	A-375	normal	nan	skin cancer	nan	NaN	A375_SKIN	DAOY_CENTRAL_NERVOUS_SYSTEM	2.496623e-07	0.007701	0.088255	nan	nan	524	human	sglacz	CRISPR	Female	A375_SKIN	0.671925	13.649916	0.464200	11.962996	72, 96	cell_line	1035	1	2919	13771.0	2.730375	40.592550	NaN
AAATGCCTCGTGGACC-1	ACH-000762	True	0.075085	YD-38	normal	nan	head and neck cancer	nan	NaN	YD38_UPPER_AERODIGESTIVE_TRACT	IGR1_SKIN	2.366136e-02	0.032628	0.158193	nan	nan	407	human	sggpx4-2	CRISPR	Male	YD38_UPPER_AERODIGESTIVE_TRACT	0.571537	7.417734	0.331906	5.552359	72, 96	cell_line	829	1	2456	10996.0	2.528192	39.841761	NaN

list(crispr_metadata["perturbation"].unique())

Since the perturbation metadata contains the guide RNA names, we model the genetic perturbations using both wetlab.GeneticTreatment and wetlab.TreatmentTarget.

treatments = [
    ("sgGPX4-1", "GPX4", "Glutathione Peroxidase 4"),
    ("sgGPX4-2", "GPX4", "Glutathione Peroxidase 4"),
    ("sgor2j2", "or2j2", "Olfactory receptor family 2 subfamily J member 2"),
    ("sgLACZ", "lacz", "beta-galactosidase control"),  # Control from E. coli
]
organism = bt.Organism.lookup().human

genetic_treatments = []
for name, symbol, target_name in treatments:
    treatment = wl.GeneticTreatment(system="CRISPR KO", name=name).save()
    if symbol != "lacz":
        gene_result = bt.Gene.from_source(symbol=symbol, organism=organism)
        gene = gene_result[0] if isinstance(gene_result, list) else gene_result
        gene = gene.save()
    else:
        gene = bt.Gene(symbol=symbol, organism=organism).save()
    target = wl.TreatmentTarget(name=target_name).save()
    target.genes.add(gene)
    treatment.targets.add(target)
    genetic_treatments.append(treatment)

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✓ created 1 Gene record from Bionty matching symbol: 'GPX4'

! record with similar name exists! did you mean to load it?

	uid	name	system	sequence	on_target_score	off_target_score	run_id	created_by_id	updated_at
id
1	ZzGBOnrjhpnd	sgGPX4-1	CRISPR KO	None	None	None	1	1	2024-10-04 09:30:11.193692+00:00

→ returning existing TreatmentTarget record with same name: 'Glutathione Peroxidase 4'

✓ created 1 Gene record from Bionty matching synonyms: 'or2j2'

! ambiguous validation in Bionty for 1 record: 'OR2J2'

Compound perturbations¶

Although the targets are known for many compounds, we skip annotating them here to keep the guide brief.

# We are using the chebi/chembl chemistry/drug ontology for the drug perturbations
chebi_source = bt.Source.filter(entity="Drug", name="chebi").one()
wl.Compound.add_source(chebi_source)
compounds = wl.Compound.public()

drug_metadata.head(3)

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	depmap_id	cancer	cell_det_rate	cell_line	cell_quality	channel	disease	dose_unit	dose_value	doublet_CL1	doublet_CL2	doublet_GMM_prob	doublet_dev_imp	doublet_z_margin	hash_assignment	hash_tag	num_SNPs	organism	perturbation	perturbation_type	sex	singlet_ID	singlet_dev	singlet_dev_z	singlet_margin	singlet_z_margin	time	tissue_type	tot_reads	nperts	ngenes	ncounts	percent_mito	percent_ribo	chembl-ID
AACTGGTGTCTCTCTG	ACH-000390	True	0.093159	LUDLU-1	normal	nan	lung cancer	µM	0.1	LUDLU1_LUNG	TE14_OESOPHAGUS	2.269468e-10	0.009426	0.403316	nan	nan	481	human	trametinib	drug	Male	LUDLU1_LUNG	0.655877	14.860933	0.462273	12.351139	24	cell_line	787	1	3045	12895.0	3.202792	24.955409	CHEMBL2103875
ATAGGCTCAGATTTCG	ACH-000444	True	0.145728	LU99	normal	2	lung cancer	µM	0.5	LU99_LUNG	MCAS_OVARY	8.562908e-04	0.010173	0.188284	nan	nan	1003	human	afatinib	drug	Male	LU99_LUNG	0.762847	10.648094	0.474590	8.164565	24	cell_line	1597	1	4763	23161.0	7.473771	18.051898	CHEMBL1173655
GCCAAATCAAGCCGTC	ACH-000396	True	0.117330	J82	normal	nan	urinary bladder carcinoma	µM	0.1	J82_URINARY_TRACT	IGR1_SKIN	6.490367e-08	0.009686	1.185862	nan	nan	647	human	dabrafenib	drug	Male	J82_URINARY_TRACT	0.651059	14.740111	0.404508	11.188513	24	cell_line	1159	1	3834	18062.0	2.762706	22.085040	CHEMBL2028663

compounds = wl.Compound.from_values(drug_metadata["perturbation"], field="name")

# The remaining compounds are not in chebi and we create records for them
for missing in [
    "azd5591",
    "brd3379",
    "control",
    "idasanutlin",
    "prexasertib",
    "taselisib",
]:
    compounds.append(wl.Compound(name=missing))
ln.save(compounds)

unique_treatments = drug_metadata[
    ["perturbation", "dose_unit", "dose_value"]
].drop_duplicates()

compound_treatments = []
for _, row in unique_treatments.iterrows():
    compound = wl.Compound.get(name=row["perturbation"])
    treatment = wl.CompoundTreatment(
        name=compound.name,
        concentration=row["dose_value"],
        concentration_unit=row["dose_unit"],
    )
    compound_treatments.append(treatment)

ln.save(compound_treatments)

Register curated artifact¶

artifact = curate.save_artifact(description="McFarland AnnData")

Show code cell output Hide code cell output

→ validating metadata using registries of instance laminlabs/lamindata

✓ var_index is validated against Gene.ensembl_gene_id

✓ depmap_id is validated against CellLine.ontology_id

✓ cell_line is validated against CellLine.name

✓ disease is validated against Disease.name

✓ organism is validated against Organism.name

✓ perturbation_type is validated against ULabel.name

✓ sex is validated against Phenotype.name

✓ time is validated against ULabel.name

✓ tissue_type is validated against ULabel.name

• path content will be copied to default storage upon `save()` with key `None` ('.lamindb/o6cCeYsvIqX81jFL0000.h5ad')

✓ storing artifact 'o6cCeYsvIqX81jFL0000' at '/home/runner/work/lamin-usecases/lamin-usecases/docs/test-perturbation/.lamindb/o6cCeYsvIqX81jFL0000.h5ad'

• parsing feature names of X stored in slot 'var'

✓    1871 unique terms (100.00%) are validated for ensembl_gene_id

✓    linked: FeatureSet(uid='OslacUxOhcbBcb9Pgawn', n=1871, dtype='float', registry='bionty.Gene', hash='J48ZrqcLN5nq8vrbkW9Oow', created_by_id=1, run_id=1)

• parsing feature names of slot 'obs'

✓    9 unique terms (25.70%) are validated for name

!    26 unique terms (74.30%) are not validated for name: cancer, cell_det_rate, cell_quality, channel, dose_unit, dose_value, doublet_CL1, doublet_CL2, doublet_GMM_prob, doublet_dev_imp, doublet_z_margin, hash_assignment, hash_tag, num_SNPs, singlet_ID, singlet_dev, singlet_dev_z, singlet_margin, singlet_z_margin, tot_reads, ...

✓    linked: FeatureSet(uid='7zpA08RLqVtG7aslqeKI', n=9, registry='Feature', hash='9-zgdakeeZjQcsHHNOznSg', created_by_id=1, run_id=1)

✓ saved 2 feature sets for slots: 'var','obs'

✓ loaded 21 Disease records matching name: 'lung cancer', 'urinary bladder carcinoma', 'colorectal cancer', 'head and neck cancer', 'brain cancer', 'skin cancer', 'gastric cancer', 'esophageal cancer', 'ovarian cancer', 'malignant pancreatic neoplasm', 'breast cancer', 'kidney cancer', 'thyroid cancer', 'uterine corpus cancer', 'liver cancer', 'prostate cancer', 'bile duct cancer', 'sarcoma', 'neuroblastoma', 'rhabdoid tumor', ...

✓ loaded 1 Disease record matching synonyms: 'pancreatic cancer'

artifact.genetic_treatments.set(genetic_treatments)
artifact.compound_treatments.set(compound_treatments)

artifact.describe()

Show code cell output Hide code cell output

Artifact(uid='o6cCeYsvIqX81jFL0000', is_latest=True, description='McFarland AnnData', suffix='.h5ad', type='dataset', size=2511528, hash='-NmtOcllwkwqByHg6iGdBA', n_observations=1000, _hash_type='md5', _accessor='AnnData', visibility=1, _key_is_virtual=True, created_at=2024-10-04 09:30:19 UTC)
  Provenance
    .storage = '/home/runner/work/lamin-usecases/lamin-usecases/docs/test-perturbation'
    .transform = 'Perturbation'
    .run = 2024-10-04 09:29:40 UTC
    .created_by = 'testuser1'
  Labels
    .genetic_treatments = 'sgGPX4-1', 'sgGPX4-2', 'sgor2j2', 'sgLACZ'
    .compound_treatments = 'trametinib', 'afatinib', 'dabrafenib', 'gemcitabine', 'navitoclax', 'bortezomib', 'brd3379', 'JQ1', 'azd5591', 'control', ...
    .organisms = 'human'
    .diseases = 'lung cancer', 'urinary bladder carcinoma', 'colorectal cancer', 'head and neck cancer', 'brain cancer', 'skin cancer', 'gastric cancer', 'esophageal cancer', 'ovarian cancer', 'malignant pancreatic neoplasm', ...
    .cell_lines = 'LUDLU-1', 'LU99', 'J82', 'HCT-15', 'YD-10B', 'SNB75', 'SK-MEL-2', 'UM-UC-1', 'AGS', 'COLO-680N', ...
    .phenotypes = 'Male', 'Female', 'Unknown'
    .ulabels = 'drug', 'CRISPR', '24', '72, 96', '3, 6, 12, 24, 48', '6', 'cell_line'
  Features
    'cell_line' = 'LUDLU-1', 'LU99', 'J82', 'HCT-15', 'YD-10B', 'SNB75', 'SK-MEL-2', 'UM-UC-1', 'AGS', 'COLO-680N', ...
    'disease' = 'lung cancer', 'urinary bladder carcinoma', 'colorectal cancer', 'head and neck cancer', 'brain cancer', 'skin cancer', 'gastric cancer', 'esophageal cancer', 'ovarian cancer', 'malignant pancreatic neoplasm', ...
    'organism' = 'human'
    'perturbation_type' = 'drug', 'CRISPR'
    'sex' = 'Male', 'Female', 'Unknown'
    'time' = '24', '72, 96', '3, 6, 12, 24, 48', '6'
    'tissue_type' = 'cell_line'
  Feature sets
    'var' = 'MAGED2', 'CPE', 'DDX43', 'SPANXA2', 'LNCPRESS1', 'TMEM175', 'None', 'MMD', 'LINC01834', 'DYNC2I1', 'CCRL2', 'MRPS6', 'KCNH1', 'TBILA', 'SLC5A7', 'GSX2', 'ATP5MG', 'TMEM190', 'GTF2H1'
    'obs' = 'tissue_type', 'perturbation_type', 'time', 'disease', 'sex', 'perturbation', 'organism', 'cell_line', 'depmap_id'